HAT1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7954-50, BS7954-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14929

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF CHIP,

All Applications: WB: 1:500~1:2000
IHC/IF: 1:50~1:200
CHIP: 1:50~1:200

Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6 have been identified as histone deacetylases.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HAT1 polyclonal antibody detects endogenous levels of HAT1 protein.

Bioworld Molecular Weight: ~ 49 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: WesternBlot (WB) analysis of HAT1 polyclonal antibody, Immunofluorescence analysis of U2OS cells, using HAT1 polyclonal antibody

Alternative Name: Histone acetyltransferase type B catalytic subunit; Histone acetyltransferase 1; HAT1; KAT1

Immunogen: Recombinant full length Human HAT1.

Conjugate: Unconjugated

Modification: Unmodified