EB3 Polyclonal Antibody

Catalogue Numbers: BS60423-50, BS60423-100

Sizes: 50µl, 100µl

Alternative Name: Microtubule-associated protein RP/EB family member 3; EB1 protein family member 3; EBF3; End-binding protein 3; EB3; RP3; MAPRE3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UPY8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: EB1 (MAPRE2, microtubule-associated protein, RP/EB family, member 2, EB2, RP1) may influence tumorigenesis of colorectal cancers and proliferative control of normal cells. EB1 may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Colorectal cancer is caused by the pathologic transformation of normal colonic epithelium to an adenomatous polyp, which can become an invasive cancer. APC (adenomatous polyposis coli) is a tumor suppressor gene, the mutation of which is one of the earliest events in colorectal carcinogenesis. A majority of the mutations result in the loss of the carboxy terminus of APC. EB1 has been shown to bind to the carboxy terminal region of APC, which implicates EB1 in APC suppression of colonic cancer. EB1 overexpression may play a role in the development of human esophageal squamous cell carcinoma (ESCC) by affecting APC function and activating the beta-catenin/TCF pathway. EB3 is related to EB1 and likewise associates with the microtubule cytoskeleton. EB3 is expressed predominantly in the central nervous system and preferentially associates with APCL.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EB3 polyclonal antibody detects endogenous levels of EB3 protein.

Bioworld Molecular Weight: ~ 32 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EB3 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human EB3.

Conjugate: Unconjugated

Modification: Unmodified