WNT16 Polyclonal Antibody

Catalogue Numbers: BS62537-50, BS62537-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UBV4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: The Wnt genes encode a family of secreted extracellular signaling glycoproteins, which function in a variety of important developmental processes such as regulation of cell growth and differentiation. Wnt proteins also play roles in carcinogenesis. Wnt-14, rather than Wnt-15, is preferentially expressed in various types of human cancer. Wnt-15 is expressed in fetal and adult kidney and is most homologous to Wnt-14. Wnt-16, another member in the Wnt family, has two mRNA isoforms, Wnt-16a and Wnt-16b. These isoforms differ in the composition of their 5'UTR and first exon, which results in differential expression. Wnt-16a is expressed only in pancreas, whereas Wnt-16b is highly expressed in adult kidney, placenta, brain, heart and spleen, but not in bone marrow. However, Wnt-16 transcripts are present in bone marrow and cell lines derived from pre-B acute lymphoblastoid leukemia patients carrying the E2A-Pbx1 hybrid gene. Thus, Wnt-16 is a downstream target of E2APbx1, and the Wnt-16-mediated autocrine growth mechanism may contribute to the development of t(1;19) pre-B acute lymphoblastoid leukemias.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: WNT16 polyclonal antibody detects endogenous levels of WNT16 protein.

Extra Notes: Western blot (WB) analysis of WNT16 polyclonal antibody at 1:500 dilution
LaneA:SP2/0 whole cell lysate
LaneB:The Spleen tissue lysate of Mouse
LaneC:The Spleen tissue lysate of Rat
LaneD:HEK293T whole cell lysate
LaneE:Panc1 whole cell lysate

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Protein Wnt-16; WNT16

Immunogen: Synthetic peptide, corresponding to Human WNT16.

Conjugate: Unconjugated

Modification: Unmodified