Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F039-48, E-BC-F039-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: FFA;NEFA

Research Areas: Metabolic Diseases;Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Fluorescence Microplate Reader

Tested Sample Types: Serum, plasma, animal tissue, cells

Assay Time: 70 min

Detection Type: Fluorescence method

Sensitivity: 0.58 μmol/L

Detection Range: 0.58-20 μmol/L

Test Principle: Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

Inter CV: 5.3

Intra CV: 4.2

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag