Cytokeratin 4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90381-50, BS90381-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P19013(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Cytokeratins are a subfamily of intermediate filament keratins that are characterized by a remarkable biochemical diversity, which is represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in isoelectric range between 4.9 and 7.8. Cytokeratin 1 has the highest molecular weight, while Cytokeratin 19 has the lowest molecular weight. The cytokeratins are divided into the type I and type II subgroups. Type II family members comprise the basic to neutral members, Cytokeratins 1-8, while the type I group comprises the acidic members, Cytokeratins 9-20. Various epithelia in the human body usually express cytokeratins which are characteristic of the type of epithelium and related to the degree of maturation or differentiation within said epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. Cytokeratin 4 is expressed in differentiated layers of the mucosal and esophageal epithelia along with Cytokeratin 13.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cytokeratin 4 polyclonal antibody detects endogenous levels of Cytokeratin 4 protein.

Bioworld Molecular Weight: 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Cytokeratin 4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Cytokeratin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CK-4, K4, KRT4, CK 4, CK-4, CK4, CYK4, Cytokeratin 4, Cytokeratin-4, Cytokeratin4, FLJ31692, K2C4, K4, Keratin 4, Keratin, Keratin type II cytoskeletal 4, Keratin-4, Keratin4, KRT 4, Krt4, type II cytoskeletal 4, Type-II keratin Kb4,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified