ADORA3 (E318) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2583-50, BS2583-100

Sizes: 50µl, 100µl

Swiss-Prot: P33765

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

Background: Adenosine is involved in a variety of processes, including the synthesis of urea, the anti-inflammatory response and the inhibition of protein synthesis The adenosine receptors, including adenosine A1-R, adenosine A2A-R, adenosine A2B-R and adenosine A3-R, are integral membrane proteins that are members of the G protein-coupled receptor family. The A1-R protein mediates ureagenesis in a partially calcium-dependent manner. Adenosine is known to mediate coronary vasodilation via the A2A-R receptor. Collagen synthesis and total protein synthesis are inhibited in certain cells by adenosine, acting via the A2B receptors. Activation of the A3-R receptor inhibits the induction of the cytokine TNFα and blocks the endotoxin CD14 receptor signal transduction pathway.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ADORA3 (E318) polyclonal antibody detects endogenous levels of Adenosine receptor A3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ADORA3 pAb at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: SHSY5Y whole cell lysate (40ug)
Lane 3: SP2/0 whole cell lysate (40ug)
Lane 4: A549 whole cell lysate (40ug)
Lane 5: Jurkat whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ADORA3 (E318) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasm and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 38 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: ADORA3; ORF Names: UNQ1931/PRO4406; Adenosine receptor A3; Adenosine A3-R

Immunogen: Synthetic peptide, corresponding to amino acids C-terminus of Human ADORA3.

Conjugate: Unconjugated

Modification: Unmodified