ERRβ Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: GCP06-50, GCP06-100

Sizes: 50µl, 100µl

Swiss-Prot: O95718

Host: Rabbit

Reactivity: Human

Applications: WB IF

Background: ERRβ/γ Antibody (E-1) is a high quality monoclonal ERRβ/γ antibody (also designated ERRβ/γ antibody) suitable for the detection of the ERRβ/γ protein of mouse, rat and human origin. ERRβ/γ Antibody (E-1) is available as both the non-conjugated anti-ERRβ/γ antibody form, as well as multiple conjugated forms of anti-ERRβ/γ antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Estrogen and progesterone receptors are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements (EREs) alters transcription of ERE-containing genes. Estrogen receptor-related proteins (ERRα, β and γ) are orphan nuclear receptors. Like estrogen receptors, ERRs bind specifically to EREs to activate reporter genes. ERRβ, also known as steroid hormone receptor ERR2 or estrogen receptor-like 2, is expressed during mammary gland development and is critical in embryo development. The loss of ERRβ results in severely impaired chorion formation leading to placental failure and embryonic death. ERRβ also potently represses the transcriptional activity of Nrf2. ERRγ, also known as ERR3, is abundantly expressed in fetal heart. The loss of ERRγ results in lactatemia and death within the first week of life.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ERRβ polyclonal antibody detects endogenous levels of ERRβ protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ERRβ polyclonal antibody at 1:1000 dilution
Lane 1: MCF-7 whole cell lysate (40ug)
Lane 2: MCF-7 nuclear lysate (20ug), Immunofluorescence analysis of MCF-7 cells using ERRβ antibody at dilution of 1:50.

Bioworld Molecular Weight: ~ 48 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Steroid hormone receptor ERR2; ERR beta-2; Estrogen receptor-like 2; Estrogen-related receptor beta; ERR-beta; Nuclear receptor subfamily 3 group B member 2; ESRRB; ERRB2; ESRL2; NR3B2

Immunogen: Synthetic peptide, corresponding to Human ERRβ.

Conjugate: Unconjugated

Modification: Unmodified