Phospho-Smad3 (Ser425) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16381-50, BZ16381-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P84022

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF,WB,IHC-F,IHC-P,ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Smad3 in variousLysates using Phospho-Smad3 antibody.

Bioworld Molecular Weight: Calculated MW: 48 kDa; Observed MW: 58 kDa

Specificity: IgG

Alternative Name: SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Immunogen: The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425. AA range:376-425

Conjugate: Unconjugated

Modification: Phosphorylated