WWOX (A7) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2426-50, BS2426-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NZC7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: WWOX (WW domain containing oxidoreductase) protein is a candidate tumor suppressor consisting of two WW domains that influence protein-protein interactions, and a short chain dehydrogenase (SDR) domain, that influences sex-steroid metabolism. Modulation of the WWOX gene influences esophageal squamous cell carcinogenesis and tumorigenicity of breast cancer cell lines MDA-MB-435 and T47D. The murine homolog WOX1 localizes in the mitochondria, and contains a mitochondrial targeting sequence mapping within the SDR domain. JNK1 can physically associate with WOX1 and sequester WOX1-dependent apoptosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: WWOX (A7) polyclonal antibody detects endogenous levels of WWOX protein.

Bioworld Molecular Weight: ~ 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of WWOX (A7) polyclonal antibody at 1:500 dilution
Lane 1: The Testis tissue lysate of Mouse (40ug)
Lane 2: The Testis tissue lysate of Rat (40ug)
Lane 3: SK-OVCAR3 whole cell lysate (40ug)
Lane 4: A2780 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of WWOX (A7) pAb in paraffin-embedded human breast carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of WWOX (A7) pAb in paraffin-embedded human colon carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of WWOX (A7) pAb in paraffin-embedded human liver carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: WW domain-containing oxidoreductase; Fragile site FRA16D oxidoreductase; WWOX; FOR; WOX1

Immunogen: Synthetic peptide, corresponding to the N-terminual of Human WWOX.

Conjugate: Unconjugated

Modification: Unmodified