Recombinant Human Neuropilin-2/NRP2 Protein (Fc Tag)(Active)

Size: 50μg

Catalogue Number: PKSH031480-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Neuropilin-2; NRP2

Target Symptom: Neuropilin-2; NP2; NPN2; PRO2714; VEGF165R2

Research Area: Neuroscience

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-hFc

Accession: NP_003863.2

Background: Neuropilin-2 (NRP-2) which is related to NRP-1, is a type I? transmembrane glycoprotein and has the structure characteristic with five main extracellular domains: two complement binding (CUB) domains, two coagulation factor V/VIII homology domains, and a MAM (meprin, tyrosine phosphatase domain) region. NRP-2 is a receptor capable of binding two disparate ligands, classⅢ semaphorins (SEMA) and vascular endothelial growth factors (VEGF), and thus regulates two diverse systems by activating cellular signaling pathways via interacting with other cell surface receptors such as VEGF receptors and plexins. NRP-2 is well known for its role in facilitating axonal guidance during the development of the neuronal system, and additionally, it is also expressed in vascular endothelial cells and lymphatic endothelium where it affects proliferation, migration, angiogenesis, as well as formation of small lymphatic vessels and capillaries. Recent study has identified NRP-2 as a polysialylated protein expressed in human dendritic cells and modulates DC-T cell Interactions. Nearly all tumor cells express neuropilins and NRP-2 is predominantly expressed in neuronal tumors and melanomas. Furthermore, it is suggested that as the specific ligand for NRP-2, SEMA 3F inhibits tumor angiogenesis and metastasis.

Activity: Measured by its binding ability in a functional ELISA. 2. Immobilized human VEGFC-His at 10μg/mL (100μL/well) can bind human NRP2-Fc, the EC50 of human NRP2-Fc is 0.1-0.5μg/mL.

Sequence: Met 1-Tyr855

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 120.7 kDa

Observed MW: 119-129 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only