CYP8B1 Polyclonal Antibody

Catalogue Numbers: BS5986-50, BS5986-100

Sizes: 50µl, 100µl

Alternative Name: 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase; CYP12

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UNU6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: CYP8B1 (sterol 12-alpha-hydroxylase) is a member of the cytochrome P450 superfamily of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. CYP8B1 is highly expressed in liver and is an important enzyme for bile acid synthesis. Specifically, CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. The gene encoding human CYP8B1 maps to chromosome 3p22.1. The CYP8B1 gene encodes a 501-amino acid protein and does not contain any introns. The CYP8B1 gene promoter is transactivated by hepatocyte nuclear factor 4a. In mice, disruption of the CYP8B1 gene prevents the synthesis of cholate, which is a primary bile acid.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP8B1 polyclonal antibody detects endogenous levels of CYP8B1 protein.

Bioworld Molecular Weight: ~ 58 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP8B1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 416-461 of Human CYP8B1.

Conjugate: Unconjugated

Modification: Unmodified