Recombinant Human 14-3-3 tau/14-3-3 theta/YWHAQ Protein (GST Tag)

Size: 100μg

Catalogue Numbers: PKSH031392-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: 14-3-3 tau; 14-3-3 theta; YWHAQ

Target Synonym: 1C5; 41701; HS1

UNIProt ID: P27348

Research Areas: Signal Transduction; Neuroscience; Stem cells

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-GST

Accession: P27348

Background: G protein-coupled receptor kinase 5, also known as G protein-coupled receptor kinase GRK5 and GRK5, is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRKs specifically phosphorylate agonist-occupied G protein-coupled receptors at the inner surface of the plasma membrane (PM), leading to receptor desensitization. GRKs utilize a variety of mechanisms to bind tightly, and sometimes reversibly, to cellular membranes. GRKs play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors. GRK5 contains oneAGC-kinase C-terminal domain, oneprotein kinase domain and oneRGS domain. GRK5 specifically phosphorylates the activated forms of G protein-coupled receptors. Phospholipid-stimulated autophosphorylation may represent a novel mechanism for membrane association and regulation of GRK5 activity. GRK5 deficiency significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. GRK5 deficiency has been linked to early Alzheimer's disease in humans and mouse models of the disease.

Sequence: Met 1-Asn 245

Purity: > 88 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 0.15m NaCl, 20mM GSH, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 54.6 kDa

Observed MW: 53 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only