PU.1 (R212) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2649-50, BS2649-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17947

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The Ets transcription factor family (Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T lineage commitment and then is dramatically absent following the β selection checkpoint.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PU.1 (R212) polyclonal antibody detects endogenous levels of PU.1 protein.

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PU.1 (R212) polyclonal antibody at 1:1000 dilution
Lane 1: JURKAT whole cell lysate (37ug)
Lane 2: THP-1 whole cell lysate (37ug)
Lane 3: The blood cell tissue lysate of Rat (36ug), Immunohistochemistry (IHC) analyzes of PU.1 (R212) pAb in paraffin-embedded human colon carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of PU.1 (R212) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of PU.1 (R212) pAb in paraffin-embedded human lung carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: SPI1; PU.1; SFPI1; Transcription factor PU.1; 31 kDa-transforming protein; SPI1

Immunogen: Synthetic peptide, corresponding to amino acids 180-230 of Human PU.1.

Conjugate: Unconjugated

Modification: Unmodified