Rpb1 (P1613) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1419-50, BS1419-100

Sizes: 50µl, 100µl

Swiss-Prot: P24928

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: POLR2A (P1613) polyclonal antibody detects endogenous levels of POLR2A protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rpb1 (P1613) polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate, Immunohistochemistry (IHC) analyzes of POLR2A (P1613) pAb in paraffin-embedded human breast carcinoma tissue.

Bioworld Molecular Weight: ~ 217, 240 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2A; POLR2

Immunogen: Synthetic peptide, corresponding to amino acids 1580-1630 of Human Rpb1.

Conjugate: Unconjugated

Modification: Unmodified