Recombinant SARS-CoV-2 Nucleocapsid Protein(Active)

Size: 50μg

Catalogue Number: PKSV030309-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Nucleocapsid

Target Symptom: SARS-CoV-2 coronavirus NP Protein; SARS-CoV-2 np Protein; SARS-CoV-2 novel coronavirus Nucleoprotein Protein

Target Species: SARS-CoV-2

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-His

UNIProt ID: P0DTC2

Accession: QHD43423.2

Background: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Activity: Immobilized 2019-nCoV NP Antibody(6G9)-Fc at 5μg/ml(100 μl/well) can bind Recombinant 2019-nCoV Nucleocapsid Protein (Mammalian)-His. The ED50 of 2019-nCoV Nucleocapsid Protein-His is 0.172 ug/ml.

Sequence: Met1-Ala419

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: Please contact us for more information.

Calculated MW: 46.4 kDa

Observed MW: 50 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only