DNA pol ε 2 Polyclonal Antibody

Catalogue Numbers: BS5700-50, BS5700-100

Sizes: 50µl, 100µl

Alternative Name: DNA polymerase epsilon subunit 2; DNA polymerase II subunit 2; DNA polymerase epsilon subunit B; POLE2; DPE2; DNA pol ε2; DNA pol εB; DNA pol ε B; DNA polε2; DNApolε2; DNA polεB; DNApolεB;

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P56282

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. Exonucleases involved in these processes include DNA polymerases, including DNA pol δ and ε. DNA pol δ consists of two subunits-p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50. DNA pol δ can be regulated by cell cycle proteins. DNA pol ε is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene. The exact reactions catalyzed by DNA pol δ and ε on leading and lagging strands have not yet been elucidated.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DNA pol ε 2 polyclonal antibody detects endogenous levels of DNA pol ε 2 protein.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DNA pol ε 2 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 144-189 of Human DNA pol ε 2.

Conjugate: Unconjugated

Modification: Unmodified