DiMethyl-Histone H3 (Lys4) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16554-50, BZ16554-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: P68431

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, ICC/IF, IP, , FC, ChIP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100 ChIP: 1/20

Background: H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 15 kDa; Observed MW: 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MethylHistone H3 in HeLa lysates using DiMethyl-Histone H3 antibody., Immunohistochemistry analysis of paraffin-embedded Human liver using MethylHistone H3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: H3K4me2; H3 histone; HIST1H3A; Histone cluster 1; H3a

Immunogen: A synthesized peptide derived from human Methyl-Histone H3 (di K4)

Conjugate: Unconjugated

Modification: Methylated