GCTM-2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66709-50, MB66709-100

Product: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.

Swiss-Prot: O00592

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB (1/1000 - 1/5000), IF/ICC (1/10 - 1/50)

Background: TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells. TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan, while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin. TRA-1-60 is also detected in the serum of patients with germ cell tumors.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GCTM-2 protein.

Bioworld Molecular Weight: ~ 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GCTM-2 expression in A549 (A) whole cell lysates., Immunofluorescent analysis of GCTM-2 staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: PCLP; PCLP1; Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GCTM-2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified