Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-R0237-24, E-CL-R0237-48, E-CL-R0237-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: NOS3; eNOS

UNIProt ID: Q62600

Target Synonyms: ECNOS; NOS III; E-NOS;

Research Areas: Cancer, Cell Biology, Cardiovascular, Metabolism, Neuroscience

Target Species: Rat

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Rat NOS3/eNOS concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 4.69 pg/mL

Detection Range: 7.81-500 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS3/eNOS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat NOS3/eNOS. You can calculate the concentration of Rat NOS3/eNOS in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Rat NOS3/eNOS in samples. No significant cross-reactivity or interference between Rat NOS3/eNOS and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag