CD180 Polyclonal Antibody

Catalogue Numbers: BS61344-50, BS61344-100

Sizes: 50µl, 100µl

Alternative Name: CD180 antigen; Lymphocyte antigen 64; Radioprotective 105 kDa protein; CD_antigen: CD180; LY64, RP105

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99467

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: RP105 is a mouse B-cell surface molecule that transmits a growth-promoting signal and is implicated in the life/death decision of B cells. RP105 has tandem repeats of a leucine-rich motif in its extracellular domain that are presumed to be involved in protein-protein interactions. The amino acid sequence of human RP105 is highly homologous to that of mouse RP105; human RP105 shares 74% identity with the mouse protein, as well as the leucine-rich motif. Surface expression of RP105 is enhanced in the presence of MD1, although this expression is restricted to CD19-positive B cells. RP105 demonstrates predominant expression on mature B cells in mantle zones; very little expression is observed in germinal centers.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD180 polyclonal antibody detects endogenous levels of CD180 protein.

Bioworld Molecular Weight: ~ 74 kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD180 polyclonal antibody at 1:1000 dilution Lane 1: HEK293T whole cell lysate(57ug) Lane 2: The spleen tissue lysate of Mouse(36ug)
Lane 3: RAW264.7 whole cell lysate(51ug)

Immunogen: A synthetic peptide corresponding to residues in Human CD180.

Conjugate: Unconjugated

Modification: Unmodified