Phospho-B-Raf-S445 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6338-50, BS6338-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P15056

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Raji cells, using Phospho-B-Raf-S445 pAb at 1:2000 dilution or BRAF antibody. Raji cells were treated by PMA/TPA for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.

Bioworld Molecular Weight: 100kDa

Specificity: Phosphorylated Antibodies

Alternative Name: BRAF; B-RAF1; B-raf; BRAF1; NS7; RAFB1

Immunogen: A synthetic phosphorylated peptide around S445 of human BRAF(NP_004324.2).

Conjugate: Unconjugated

Modification: Phosphorylated