Recombinant Mouse RANKL/TNFSF11 Protein (His Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSM041165-10, PKSM041165-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: RANKL; TNFSF11

Target Symptom: Tumor necrosis factor ligand superfamily member 11; Tnfsf11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD254

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-6His

UNIProt ID: O35235

Accession: O35235

Background: Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor (TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such as thymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation.

Activity: Immobilized Human OPG-Fc(Cat: PKSH033124) at 2μg/ml(100 μl/well) can bind Mouse RANKL-His. The ED50 of Mouse RANK L-His is 2. 44ug/ml.

Sequence: Ala73-Asp316

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 28.5 kDa

Observed MW: 30-35 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only