COX2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90326-50, BS90326-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35354(Human) Q05769(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200

Background: Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutively expressed in most tissues and is thought to serve in general “housekeeping” functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: COX2 polyclonal antibody detects endogenous levels of COX2 protein.

Bioworld Molecular Weight: 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of COX2 on different lysates using anti-COX2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: THP-1, ICC staining COX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: COX-2, PGH synthase 2, PGHS-2, PTGS2, COX 2, COX-2, COX2, Cyclooxygenase 2, Cyclooxygenase 2b, Cyclooxygenase, Cyclooxygenase-2, Cyclooxygenase2, EC 1.14.99.1, fj02a10, Glucocorticoid-regulated inflammatory cyclooxygenase, Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase, GRIPGHS, hCox 2, Macrophage activation-associated marker protein P71/73, OTTHUMP00000033524, PES-2, PGG/HS, PGH synthase 2, PGH2, PGHS 2, PGHS-2, PGHS2, PHS 2, PHS II, PHS2, Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), Prostaglandin endoperoxide synthase 2, Prostaglandin G/H synthase 2, Prostaglandin G/H synthase 2 precursor, Prostaglandin G/H synthase and cyclooxygenase, Prostaglandin G/H synthase, Prostaglandin H2 synthase 2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), Prostaglandin-endoperoxide synthase 2, PTGS2, ptgs2a, TIS10, TIS10 protein, unp1239, wu:fj02a10,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified