Recombinant Human POLD2 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032361-10, PKSH032361-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: POLD2

Target Synonym: DNA Polymerase Delta Subunit 2; DNA Polymerase Delta Subunit p50; POLD2

UNIProt ID: P49005

Research Areas: epigenetics and nuclear signal

Target Species: Human

Expression Host: E.coli

Fusion Tag: C-6His

Accession: P49005

Background: DNA Polymerase Delta Subunit 2 (POLD2) belongs to the DNA polymerase delta/II small subunit family. POLD2 can form a heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. POLD2 possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. POLD2 is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA).

Sequence: Met 1-Pro469

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 52.3 kDa

Observed MW: 56 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only