Skp1 p19 (P72) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1694-50, BS1694-100

Sizes: 50µl, 100µl

Swiss-Prot: P63208

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Skp1 p19 (P72) polyclonal antibody detects endogenous levels of Skp1 p19 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SKP1 pAb at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (40ug)
Lane 2: A2780 whole cell lysate (40ug)
Lane 3: Hela whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Skp1 p19 (P72) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 19 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: p19A; RNA polymerase II elongation factor-like protein; Organ of Corti protein 2; OCP-2; OCP-II; Transcription elongation factor B; SIII; SKP1; EMC19; OCP2; SKP1A; TCEB1L

Immunogen: Synthetic peptide, corresponding to amino acids 40-90 of Human Skp1 p19.

Conjugate: Unconjugated

Modification: Unmodified