SCT Polyclonal Antibody

Catalogue Numbers: BS60409-50, BS60409-100

Sizes: 50µl, 100µl

Alternative Name: Secretin; SCT

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09683

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Secretin is a 27-amino acid hormone produced by specific endocrine cells, S cells, located in the mucosa of the proximal small intestine. Secretin is known to be a potent stimulus for the secretion of bicarbonate-rich pancreatic juice. Secretion of secretin is stimulated by the presence of either acidic pH or fatty acids in the duodenum. Secretin is synthesized as a larger precursor. The deduced amino acid sequence includes a signal peptide, an amino-terminal peptide, secretin itself, and a 72-amino acid carboxy-terminal peptide. Secretin stimulates ductal bile secretion by directly interacting with cholangiocytes. It stimulates exocytosis in cholangiocytes, which transport water mainly via the water channel aquaporin-1. Secretin deficiency may be implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. The gene which encodes secretin maps to human chromosome 11p15.5.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SCT polyclonal antibody detects endogenous levels of SCT protein.

Bioworld Molecular Weight: ~ 278 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SCT polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human SCT.

Conjugate: Unconjugated

Modification: Unmodified