Cytokeratin 19 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90379-50, BS90379-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08727 Human;P19001 Mouse

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:2,000-1:20,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. For example, many types of cancer cells express Cytokeratin 19 (CK19), an epithelial cytoskeletal protein within the suprabasal squamous epithelium. Cytokeratin 19 is a specific marker of moderate to severe dysplasia and carcinoma in situ in oral cavity squamous epithelium, and measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma. Cytokeratin 19 fragment levels in serum have been documented as a marker for lung cancer. Clinical investigations have suggested that serum CYFRA 21-1, a fragment of Cytokeratin 19, may be among the most useful tumor markers.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: Cytokeratin 19 polyclonal antibody detects endogenous levels of Cytokeratin 19 protein.

Bioworld Molecular Weight: 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cytokeratin 19 on rat lung tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:20,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat lung tissue lysate
Lane 2: MCF-7 cell lysate
Lane 3: Mouse lung tissue lysate, ICC staining Cytokeratin 19 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 19 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Alternative Name: CK-19, K19, KRT19, 40 kDa keratin intermediate filament, CK 19, CK-19, CK19, Cytokeratin 19, Cytokeratin-19, K19, K1C19, K1CS, Keratin 19, Keratin type I 40 kD, Keratin type I 40kD, Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, Keratin, type I, 40 kd, Keratin-19, KRT19, MGC15366 antibody

Immunogen: Recombinant protein within Human Cytokeratin 19 aa 100-350.

Conjugate: Unconjugated

Modification: Unmodified