EPCAM Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9010-50, MB9010-100

Product: Mouse IgM, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16422

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, ICC IHC FC

All Applications: WB: 1:1000-1:2000 ICC: 1:100-1:200
IHC: 1:200-1:500 FC: 1:100-1:200

Background: The epithelial cell adhesion molecule Ep-CAM, which is also design atedtumor-associated calcium signal transducer 1 and MK-1, is a monomeric membrane glycoprotein that is expressed in most normal human epithelium and in most carcinomas. The human Ep-CAM gene encodes a 314 amino acid protein that is expressed as two forms, a 40 kDa major form and a 42 kDa minor form, which are reduced to 35 kDa upon treatment with the aminoglycosylation inhibitor tunicamycin. Ep-CAM is overexpressed in a variety of carcinomas and is, therefore, a potential target for the visualization and therapy of human solid tumours. Ep-CAM contains an extracellular domain containing two epidermal growth factor-like repeats, followed by a cysteinepoor region, which are necessary for the adhesion properties of the molecule.

Purification and Purity: ProL affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of EPCAM and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EPCAM Mouse mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: MCF7 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin., ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD326; EPCAM; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1

Immunogen: This antibody is produced by immunizing mice with recombinant protein corresponding to a region of EpCAM.

Conjugate: Unconjugated

Modification: Unmodified