Phospho-BRCA1 (Ser1423) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16480-50, BZ16480-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P38398

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background: E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (PubMed:20160719).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 208 kDa; Observed MW: 208 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide , using BRCA1 (Phospho-Ser142antibody, Immunohistochemical analysis of paraffin-embedded Human tonsils using Phospho-BRCA1 antibody. Sample with blocking peptide on the right. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: BRCA1; RNF53; Breast cancer type 1 susceptibility protein; RING finger protein 53

Immunogen: The antiserum was produced against synthesized peptide derived from human BRCA1 around the phosphorylation site of Ser1423. AA range:1391-1440

Conjugate: Unconjugated

Modification: Phosphorylated