EasyStep Human fT3 (Free Triiodothyronine) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK048ES-48, ELK048ES-96, ELK048ES-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

Assay Type: Competitive Inhibition

Sensitivity: 0.31 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma

Assay Length: 1.5h

Research Areas: Endocrinology; Autoimmunity;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human fT3 antigen, and the Human fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only