Rat NPT (Neopterin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0783-48, ELK0783-96, ELK0783-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Neopterin

Assay Type: Competitive Inhibition

Sensitivity: 0.27 ng/mL

Standard: 50 ng/mL

Detection Range: 0.78-50 ng/mL

Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Cytokine; Infection immunity;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NPT in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only