LPCAT1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS70662-50, BS70662-100

Swiss-Prot: Q8NF37

Host: Rabbit

LPCAT1 Polyclonal Antibody-BS70662

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:1000 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family of proteins. The encoded enzyme plays a role in phospholipid metabolism, specifically in the conversion of lysophosphatidylcholine to phosphatidylcholine in the presence of acyl-CoA. This process is important in the synthesis of lung surfactant and platelet-activating factor (PAF). Elevated expression of this gene may contribute to the progression of oral squamous cell, prostate, breast, and other human cancers. [provided by RefSeq, Sep 2016]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 59KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of variousLysates, using LPCAT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of MCF7 cells using LPCAT1 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: LPCAT1; AGPAT10; AGPAT9; AYTL2; LPCAT-1; PFAAP3; lpcat; lysoPAFAT

Immunogen: Recombinant fusion protein of human LPCAT1(NP_079106.3).

Modification: Unmodified

Conjugate: Unconjugated