Recombinant Mouse CD80/B7-1 Protein (His Tag)(Active)

Size: 100μg

Catalogue Number: PKSM040679-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD80; B7-1

Target Symptom: T-lymphocyte activation antigen CD80; Activation B7-1 antigen; B7; CD80; Cd28l; Ly-53; Ly53; MIC17; TSA1

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-His

UNIProt ID: Q00609

Accession: Q00609-1

Background: The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Activity: Measured by its ability to bind recombinant mouse CD28 in a functional ELISA.

Sequence: Met 1-Lys 245

Purity: > 97 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 25.1 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only