p53 (Phospho-T55) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94087-50, BS94087-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04637(Human)

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB:1:1,000-1:2,000

Background: p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-p53 (T55) on human skinLysates using anti-Phospho-p53 (T55) antibody at 1/1,000 dilution.

Bioworld Molecular Weight: 53 kDa

Specificity: p53 (Phospho-T55) polyclonal antibody detects endogenous levels of p53 protein only when phosphorylated at T55.

Alternative Name: TP53, Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53, p53 tumor suppressor, P53, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr55 of human p53

Conjugate: Unconjugated

Modification: Phosphorylation