DDX3X Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-61035-60, E-AB-61035-120, E-AB-61035-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: DDX3X

Target Synonym: DDX3X; CAP-Rf; DBX; DDX14; DDX3; HLP2; MRX102

Research Areas: Cancer, Epigenetics and Nuclear Signaling, Developmental Biology, Stem Cells

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: O00571

Background: The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human DDX3X (NP_001347.3).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200

Calculated MW: 71kDa/73kDa

Observed MW: 80kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only