Human Anti-TTN (Anti-Titin Antibody) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0760-48, ELK0760-96, ELK0760-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CMD1G; CMH9; CMPD4; HMERF; LGMD2J; TMD; Cardiomyopathy,Dilated 1G (Autosomal Dominant)

Assay Type: Competitive Inhibition

Sensitivity: 0.14 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: cell lysates, cell culture supernates and other biological fluids

Assay Length: 2.5h

Research Areas: Developmental science; Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-TTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-TTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-TTN in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only