Cdk2 (Phospho-Y15) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94063-50, BS94063-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P24941(Human) P97377(Mouse) Q63699(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB:1:1,000
IHC:1:50-1:200

Background: In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cdk2 (Phospho-Y15) polyclonal antibody detects endogenous levels of Cdk2 protein only when phosphorylated at Y15.

Bioworld Molecular Weight: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Cdk2(Y15) on different lysates using anti-Phospho-Cdk2(Y15) antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse spleen
Lane 3: NIH/3T3, Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.

Alternative Name: CDK2, Cdc2 related protein kinase, cdc2-related protein kinase, CDC28, CDC2A, Cdk 2, CDK1, CDK2, CDK2, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, Cyclin dependent kinase 2, cyclin dependent kinase 2-alpha, Cyclin-dependent kinase 2, kinase Cdc2, MPF, p33 protein kinase, p33(CDK2),

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr15 of human Cdk2.

Conjugate: Unconjugated

Modification: Phosphorylation