CD3e Polyclonal Antibody

Catalogue Numbers: BS62211-50, BS62211-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07766

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery. This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline-region is exposed and then associates with the adapter protein Nck.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD3e polyclonal antibody detects endogenous levels of CD3e protein.

Extra Notes: Western blot analysis of extracts of Jurkat and MOLT-4 cell line, using CD3e polyclonal antibody.

Bioworld Molecular Weight: ~ 21 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen=CD3e; CD3E; T3E

Immunogen: Synthetic peptide, corresponding to Human CD3e.

Conjugate: Unconjugated

Modification: Unmodified