Recombinant Human Semaphorin 4D/SEMA4D Protein (Fc Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033023-10, PKSH033023-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: SEMA4D; CD100

Target Symptom: Semaphorin-4D; A8; BB18; GR3; CD100; C9orf164; CD100; coll-4; M-sema-G; SEMAJ

Research Area: Neuroscience; immunology

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-Fc

UNIProt ID: Q92854

Accession: Q92854

Background: Semaphorin-4D is also known as A8; BB18; GR3; CD100. Semaphorin-4D belongs to the semaphorin family containing 1 Ig-like C2-type domain; 1 PSI domain and 1 Sema domain. It is the cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. It promotes the migration of cerebellar granule cells and of endothelial cells; regulates dendrite and axon branching and morphogenesis. Semaphorin-4D Plays a role in the immune system; Promotes signaling via SRC and PTK2B/PYK2; which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade.

Activity: Immobilized Mouse PLXDC2 -His(Cat: PKSH032037) at 2μg/ml(100 μl/well) can bind Human SEMA4D-Fc.

Sequence: Met22-Arg734

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 106.4 kDa

Observed MW: 130-170 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only