CHAT Polyclonal Antibody Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Sizes: 50µl, 100µl Catalogue Numbers: BS80072-50, BS80072-100 Swiss-Prot: P28329 Host: Rabbit CHAT Polyclonal Antibody-BS80072 Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Reactivity: Human, Mouse, Rat Applications: WB, IF/ICC All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 Background: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer' s disease. Polymorphisms in this gene have been associated with Alzheimer' s disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Specificity: Unmodification Bioworld Molecular Weight: 70kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell line, using CHAT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s., Immunofluorescence analysis of Rat brain using CHAT antibody at dilution of 1:100. Blue: DAPI for nuclear staining. Alternative Name: CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6 Immunogen: Recombinant fusion protein of human CHAT(NP_001136401.1). Modification: Unmodified Conjugate: Unconjugated