DGCR8 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10830-50, MB10830-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q8WYQ5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20

Background: Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 86 kDa; Observed MW: 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of DGCR8 in K562 using DGCR8 antibody, and DAPI., Western blot analysis of DGCR8 in K562, C6, 3T3 lysates using DGCR8 antibody.

Alternative Name: DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha

Immunogen: Recombinant protein of human DGCR8

Conjugate: Unconjugated

Modification: Unmodified