ATG2B (D2078) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP0776-50, AP0776-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96BY7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, IHC

All Applications: WB: 1:500~1:1000
IP: 1:50~1:200
IHC: 1:50~1:200

Background: ATG2B,required for both autophagosome formation and regulation of lipid droplet morphology and dispersion. Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATG2B (D2078) polyclonal antibody detects endogenous levels of ATG2B protein.

Bioworld Molecular Weight: ~ 300 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATG2B (D2078) polyclonal antibody at 1:500 dilution
Lane 1: Myla2059 whole cell lysate (40ug)
Lane 2: PC3 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: C6 whole cell lysate (40ug), Immunoprecipitation of 293T cell lysates using ATG2B (D2078) pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using ATG2B (D2078) pAb #AP0776., Immunohistochemistry of paraffin-embedded Human Ovarian cancer using ATG2B antibody at dilution of 1:50.

Alternative Name: Autophagy-related protein 2 homolog B; ATG2B

Immunogen: Synthetic peptide, corresponding to Human ATG2B.

Conjugate: Unconjugated

Modification: Unmodified