Cathepsin L HC Polyclonal Antibody

Catalogue Numbers: BS62559-50, BS62559-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07711

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsin L (also designated major excreted protein, MEP or CATL) is a member of the peptidase C1 family and has been identified as a protein that is most closely related to cathepsin H. It is a lysosomal cysteine proteinase that mediates intracellular protein catabolism for collagen, elastin and å-1 protease inhibitor. Cathepsin L is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. Transformed mouse fibroblasts stimulated by growth factors or tumor promoters secrete a form of cathepsin L.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cathepsin L HC polyclonal antibody detects endogenous levels of Cathepsin L HC protein.

Extra Notes: Western blot (WB) analysis of Cathepsin L HC polyclonal antibody at 1:500 dilution
LaneA:H9C2 whole cell lysate
LaneB:MEF whole cell lysate
LaneC:K562 whole cell lysate
LaneD:Hela whole cell lysate

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Cathepsin L1; Cathepsin L; Major excreted protein; MEP; CTSL; CTSL1

Immunogen: Synthetic peptide, corresponding to Human Cathepsin L HC.

Conjugate: Unconjugated

Modification: Unmodified