Glutamine (Gln) Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K853-M-48, E-BC-K853-M-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: Gln

Research Areas: Amino Acids and Proteins; Cuproptosis

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: serum (plasma) , animal, plant tissue

Assay Time: 70 min

Detection Type: Colorimetric method

Sensitivity: 0.036 mmol/L

Detection Range: 0.036–2.0 mmol/L

Test Principle: Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.

Inter CV: 4.7

Intra CV: 3.2

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag