Recombinant Human FGF-6 protein(N-His)(active) Sizes: 20μg, 100μg Catalogue Numbers: PKSH034151-20, PKSH034151-100 Citations, Manuals and MSDS Available upon request. Abbreviation: FGF-6 Target Symptom: HBGF-6; HST-2 Target Species: Human Expression Host: E.coli Application: Cell culture Fusion Tag: N-His UNIProt ID: P10767 Accession: P10767 Background: FGF-6 belongs to the fibroblast growth factor (FGF) family. Members of this family possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF6 plays an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis. It is also required for normal muscle regeneration. FGF6 gene displayed oncogenic transforming activity when transfected into mammalian cells. Activity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 0.1 ng/mL. The specific activity of recombinant human FGF-6 is > 1 x 10⁷IU/mg. Sequence: MALGQKLFITMSRGAGRLQGTLWALVFLGILVGMVVPSPAGTRANNTLLDSRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYCNVGIGFHLQVLPDGRISGTHEENPYSLLEISTVERGVVSLFGVRSALFVAMNSKGRLYATPSFQEECKFRETLLPNNYNAYESDLYQGTYIALSKYGRVKRGSKVSPIMTVTHFLPRI Purity: > 98 % as determined by reducing SDS-PAGE. Formulation: Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. Reconstitution: Please refer to the printed manual for detailed information. Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method. Calculated MW: 23.73 kDa Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. Shipping: This product is provided as lyophilized powder which is shipped with ice packs. Research Use Only