Human 5-MTHF (5-Methyltetrahydrofolate) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0833-48, ELK0833-96, ELK0833-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: L-5-MTHF; 5-MTHF;

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction; Reproductive science; Genetic science; Nutrition metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5-MTHF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5-MTHF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5-MTHF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5-MTHF in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P49914

Research Use Only