MARK3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90825-50, BS90825-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P27448 Human;Q03141 Mouse;Q8VHF0 Rat

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, ICC/IF IHC FC

All Applications: WB:1:500
IP:1:10-1:50
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: MARK3 (MAP/microtubule affinity-regulating kinase 3), also known as C-TAK1 (Cdc25C-associated protein kinase 1), EMK-2 (ELKL motif kinase 2), protein kinase STK10 or serine/threonine-protein kinase p78, is a 753 amino acid peripheral membrane protein that phosphorylates Cdc25C on Serine 216 and is ubiquitously expressed in various human tissue and cell lines. Existing as six alternatively spliced isoforms, MARK3 belongs to the Ser/Thr protein kinase family and the protein kinase superfamily. MARK3 has been suggested to mediate the binding of the 14-3-3 proteins through its kinase activity and acts as a negative regulator of mitosis. The gene encoding MARK3 maps to human chromosome 14q32.3 and mouse chromosome 12 F1.

Purification and Purity: Protein A purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: MARK3 polyclonal antibody detects endogenous levels of MARK3 protein.

Bioworld Molecular Weight: 84 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MARK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: rat brain tissue lysate
Lane 2: A431 cell lysate
Lane 3: 293 cell lysate, Immunocytochemistry staining MARK3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MARK3 monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Alternative Name: cTAK1, EMK-2, Par-1a, MARK3, CTAK1, EMK2, C-TAK1, Cdc25C associated protein kinase 1, Cdc25C-associated protein kinase 1, cTAK1, ELKL motif kinase 2, EMK-2, Emk2, ETK 1, KIAA4230, KP78, MAP microtubule affinity regulating kinase 3, MAP/microtubule affinity-regulating kinase 3, Mark3, MARK3, Par 1a, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine threonine protein kinase p78, Serine/threonine-protein kinase p78, SerThr protein kinase PAR 1 antibody

Immunogen: Recombinant protein within human Mark3 aa 600-750.

Conjugate: Unconjugated

Modification: Unmodified