DNA Ligase I (E139) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2528-50, BS2528-100

Sizes: 50µl, 100µl

Swiss-Prot: P18858

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: DNA Ligase I (E139) polyclonal antibody detects endogenous levels of DNA Ligase I protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DNA Ligase I (E139) pAb at 1:500 dilution
Lane 1: K562 whole cell lysate (40ug)
Lane 2: L02 whole cell lysate (40ug)
Lane 3: Jurkat whole cell lysate (40ug)
Lane 4: A549 whole cell lysate (10ug), Immunohistochemistry (IHC) analyzes of DNA Ligase I (E139) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 102 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA ligase 1; DNA ligase I; DNLI1; LIG 1; lig1; Ligase I DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 1;

Immunogen: Synthetic peptide, corresponding to amino acids 110-160 of Human DNA Ligase I.

Conjugate: Unconjugated

Modification: Unmodified