GSDMD (Full Length+N terminal) Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS79581-50, BS79581-100

Swiss-Prot: P57764

Host: Rabbit

GSDMD (Full Length+N terminal) Polyclonal Antibody-BS79581

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 29KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of THP-1, using GSDMD antibody at 1:400 dilution.THP-1 cells were treated by PMA/TPA at 37℃ for overnight and LPS at 37℃ for 6 hours
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of HeLa cells using GSDMD Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: GSDMD; DF5L; DFNA5L; FKSG10; GSDMDC1

Immunogen: Recombinant fusion protein mouse Cleaved GSDMD(NP_081236.1).

Modification: Unmodified

Conjugate: Unconjugated