Recombinant Human DKK1/Dkk-1 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033697-10, PKSH033697-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: DKK1; Dkk-1

Target Symptom: Dickkopf-related protein 1; Dickkopf-1; Dkk-1; SK

Research Area: Cardiovascular; Developmental Biology; immunology; Stem cells

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: N-His

UNIProt ID: O94907

Accession: O94907

Background: Dickkopf-related protein 1(DKK-1), is a member of the dickkopf family. DKK1 secreted proteins with two cysteine-rich domains separated by a linker region. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Sequence: Thr32-His266

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 27 kDa

Observed MW: 42-47 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only